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Halo formation over time. This image is representative of the appearance of halo-forming and non-halo-forming variants in general. Libraries were plated on top of LB plus kan-VRE-IPTG plates. (A) At approximately 16 to 18 h following incubation at 37°C, discernible halos appeared around catalytically active variants (green arrows) and not around catalytically inactive variants (red arrows). (B) At longer times (>18 h), the halo radii continued to grow.
