FIG 6.

Active fraction of variants in library as a function of average ΔE. The difference between the statistical fitness of each variant (using MSA group Firmicutes_stringent-2k) and the WT, ΔE, is plotted against the active fraction of classified mutants in a library. The central square is the median ΔE value and the left- and rightmost vertical lines represent the 25th (Q₁) and 75th (Q₃) percentiles. Whiskers extend to the most extreme points not considered outliers; outliers are defined as values less than Q₁ − W_M(Q₃ − Q₁) or greater than Q₃ + W_M(Q₃ − Q₁), where W_M is the maximum whisker length.