FIG 7.

Average active fractions for a sequence based on the amino acid at the specified position. For each position of interest, the amino acid at that site is related to the active fraction of sequences having that particular mutation. The total number of sequences considered in the calculation of the active fraction value is given in the top-right corner of each cell. Note that this is based on sequence data for single, double, and triple mutants across libraries mutating redundant positions and is therefore not a canonical heat map of independent mutation outcomes.