TABLE 2.
Library no. | Diversified positions | No. of double mutants |
Total no. |
||
---|---|---|---|---|---|
Active (% of total) | Inactive | Active (% of total) | Inactive | ||
1 | 33, 40 | 184 (94) | 11 | 211 (95) | 11 |
2 | 40, 47 | 83 (98) | 2 | 114 (98) | 2 |
3 | 45, 87 | 53 (82) | 12 | 67 (84) | 13 |
4 | 32, 38 | 96 (96) | 4 | 109 (96) | 4 |
5 | 33, 45 | 92 (100) | 0 | 114 (100) | 0 |
6 | 47, 91 | 24 (100) | 0 | 45 (98) | 1 |
7 | 34, 35 | 18 (90) | 2 | 36 (95) | 2 |
8 | 74, 83 | 12 (21) | 46 | 22 (30) | 51 |
9 | 33, 40, 87a | 74b (96) | 3 | 313 (95) | 15 |
Specific mutation I87L, not implementation of a randomized codon, at this site.
Triple mutants.