TABLE 4.

Information for purified variants and the WT

Variant	Halo forming?	Library	Mutation(s)	−ΔOD600/min/μg
WT	Yes	NAa	NA	0.048 ± 0.007
1	Yes	2	T40P, N47V	0.048 ± 0.006
2	Yes	NA	R17L, T40L	NA
3	Yes	3	M45E, I87D	NA
4	Yes	4	N32G, E38T	0.025 ± 0.004
5	Yes	5	S33P, M45W	0.041 ± 0.003
6	Yes	6	N47A, V91P	0.028 ± 0.003
7	Yes	7	T34S, A35V	0.087 ± 0.013
8	Yes	1	T40A	0.046 ± 0.003
9	Yes	1	S33G, T40S	0.022 ± 0.003
10	Yes	9	S33G, T40E, I87L	0.043 ± 0.003
	No	NA	Uncut vector	NA
11	No	3	M45P, I87G	NA
	No	NA	Uncut vector	NA
	No	NA	Uncut vector	NA
12	No	7	A35G, T34T	NA
	No	NA	Uncut vector	NA
13	No	8	A74R, N83M	0.005 ± 0.004
14	No	8	A74T, N83Y	0.008 ± 0.004

^aNA, not applicable.