FIG 3.

DUSP6 and DUSP8 expression reduction caused by EBV LMP1. (A) EBV-negative Akata and BJAB lymphoma cells were infected with vector control lentivirus (pSIN) or lentiviruses that overexpressed LMP1, LMP2A, or Zta. DUSP6, DUSP8, LMP1, LMP2A, and Zta transcripts were analyzed by RT-PCR. β-Actin was detected as an internal control. (B) BJAB cells were electroporated with EBNA1, EBNA2, or EBNA3C. DUSP6, DUSP8, EBNA1, EBNA2, and EBNA3C transcripts were analyzed by RT-PCR. β-Actin was detected as an internal control. (C) BJAB cells were infected with LMP1 lentiviruses with increasing multiplicities of infection. Protein expression of DUSP6, DUSP8, and LMP1 was detected by Western blotting. β-Actin served as an internal control. (D) LCLs were infected with lentivirus containing shRNA targeting luciferase or LMP1. Data represent protein expression of DUSP6, DUSP8, and LMP1. β-Actin was detected as an internal control.