FIG 4.

Effects of inhibitors on relocalization of ATI mRNA around inclusions. HeLa cells were infected with vATI-HA.ΔA26 and at 6 h were incubated with puromycin for 30 min. The cells were then washed and incubated without puromycin for 3 h without drug (no drug) or with actinomycin D (Actino), cycloheximide (Cyclo), nocodazole (Noco), or latrunculin (Latrun). The cells were then fixed and probed with fluorescent-labeled antisense ATI deoxyoligonucleotide probes to detect ATI mRNA, with anti-HA antibody and secondary fluorescent antibody to detect ATI protein, and with DAPI to detect DNA. Z-sections of typical cells are shown.