FIG 9.

Cloning strategy. The amino acid sequences of extracellular domains (exodomains) of the FUS4 and FUS8 glycoproteins (top), depicted here with their signal peptides (red), transmembrane region, and intracellular regions, were selected for gene synthesis. The synthetic constructs (blue arrow, middle) were bracketed by BamHI restriction enzyme sites. A third synthetic construct (red arrows, bottom), comprising a baculovirus signal sequence, an internal BamHI restriction enzyme site, a c-myc tag, and a GST tail, flanked by attB recombination sites, was cloned into pDONR221 before two variants were constructed. One variant had the FUS4 exodomain inserted into the BamHI site, and the other had the FUS8 exodomain inserted into the BamHI site.