FIG 1.

Localization of ICP22 and Hsc70 in HSV-infected cells. (A) Vero cells were infected with TF22 (mutant virus with FLAG-tagged ICP22 introduced into the HSV genome) at an MOI of 10. At 2, 4, 8, and 16 h postinfection, the cells were fixed, permeabilized, and labeled with rabbit anti-FLAG and rat anti-Hsc70. Imaging was performed at a magnification of ×2. (B) Vero cells were infected with TF22 (FLAG-tagged ICP22 virus) and d22LacZ (ICP22-null virus) at an MOI of 10. Cells were collected and lysed 10 h postinfection, and immunoprecipitation was performed with mouse anti-FLAG. The cell lysates and pulldown samples were probed with mouse anti-FLAG, rat anti-Hsc70, and mouse anti-ICP4.