FIG 3.

ICP22 is sufficient to recruit Hsc70. (A) Vero cells were transfected with 1 μg of FLAG-ICP22. After 16 h of protein expression, the cells were fixed, permeabilized, and labeled with rabbit anti-FLAG and rat anti-Hsc70 antibodies. (B) Vero cells were transfected with 500 ng of model misfolded protein GFP170* and 500 ng of FLAG-ICP22. After 16 h of protein expression, the cells were fixed, permeabilized, and labeled with rabbit anti-FLAG and rat anti-Hsc70 antibodies. GFP was detected by excitation using a 488-nm laser. (C) Vero cells were transfected as in panel B and labeled with rabbit anti-FLAG antibody. GFP was detected by excitation using a 488-nm laser.