Increased levels of metabolites involved in glutaminolysis in MDV-infected cells. GSxMS analysis of relative levels of characterized metabolites from mock-infected (control) and MDV-infected (RB1B) CEFs are shown at 48 hpi and 72 hpi. Box and whisker plots showing minimum and maximum relative levels of named metabolites either (A) significantly increased, (B) significantly decreased, or (C) showed no change as a result of MDV infection. Nonparametric Wilcoxon tests (Mann-Whitney) were used to assess normal distribution and test significance, with the results shown as mean ± SD. * (P = 0.01) and ** (P = 0.001) indicate a statistically significant difference compared to the control. NS indicates no significant difference. The experiment was performed in biological triplicates with six technical replicates per biological replicates. Con, control; Inf, infected.