FIG 3.

α-ketoglutarate rescues MDV replication in low-glutamine conditions. MDV-infected CEFs were cultured in complete medium (2 mM glutamine, 6.74 mM glucose) or low glutamine (200 μM) with or without α-ketoglutarate (α-KG) (7 mM). CEFs were infected with RB1B (100 PFU) and 72 hpi; (A) MDV plaque size and (B) MDV titers were determined. Plaque sizes are shown as box plots with minimums and maximums (20 plaques were measured in each well). (C) Representative plaque images are shown. Scale bar, 1,000 μm. All experiments were performed in triplicate, and data are representative of 3 independent experiments.