FIG 7.

Gating strategy for infected cell elimination assay. Cells are gated on forward scatter and side scatter (FSC and SSC, respectively), followed by distinguishing viable cells using NFL-1 (a dead cell marker) and TFL-4 (target cell marker). Target cells are then gated on CD4 and p24. Mock-infected cells are used to determine positioning of the gate for p24⁺. The proportion of p24⁺, p24⁺ CD4⁺, and p24⁺ CD4⁻ target cells in the presence of effectors only, effectors plus negative control, or effectors plus sample was determined.