TABLE 1.
Primer sets used for the specific quantification of gRNA and sgmRNA by RT-PCR
| Primer name | Primer sequence (5′ to 3′) | Remark |
|---|---|---|
| 481F | GCCTCAACTATGATGGCATT | gRNA |
| 620R | AGAGCACTCCCGAAGAGGAC | |
| 21886R | GTCTAAACCCATTTCCACA | sgmRNA1 (NS2a) |
| 19 F | TCCGTACGTACCCTCTCAACTCTAA | |
| 24031R | TCTCAGTGCTAATGCTTGGA | sgmRNA2 (S) |
| 19 F | TCCGTACGTACCCTCTCAACTCTAA | |
| 28397R | GCATCATCACATGCCAAAT | sgmRNA3 (ORF5) |
| 19 F | TCCGTACGTACCCTCTCAACTCTAA | |
| 29091R | GGCTCGTGTAACCGAACTGT | sgmRNA4 (M) |
| 19 F | TCCGTACGTACCCTCTCAACTCTAA | |
| GAPDH_F | AGCCACATCGCTCAGACAC | Internal control |
| GAPDH_R | GCCCAATACGACCAAATCC | |
| 27379F | TGGAGAATGGAAGTTCACAGG | Viral mRNA (S) |
| 27501R | GTCGGGTGGATTAGGTATTGAAG |