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. 2020 Jan 28;10:1579. doi: 10.3389/fphar.2019.01579

Figure 5.

Figure 5

Adoptive transfer K313 modified DCregs loaded with MOG35-55 into the EAE mice ameliorated the development of disease. The 16 μM K313 modified DCregs loaded or unloaded with MOG35-55 (20 μg/ml) were transferred into the EAE mice by intravenous injection (1 × 106 cells/mouse) at day 10, 13, and 16 postimmunization. The positive control group were given 10–8 M 1,25-Dihydroxyvitamin D3 induced DCregs loaded with antigen. The clinical scores were made according to Benson score. On day 20, the five groups of mice were perfused. After spinal cord sample fixation, paraffin embedding, slicing and dewaxing hydration, HE and LFB staining were performed. (A) The mean clinical scores of five groups of mice. The experiment was performed twice, the total number of animals in each group was 8 (n = 8). (B) Quantitative comparison of the extent of demyelinated area in different animal groups. 24 sections from each group were analyzed by ImageJ software for assessing the myelination intensity and the extent of demyelinated area. (*P < 0.05, **P < 0.01 versus the untreated group). (C) Histological examinations staining examined the extent of cellular infiltration. (D) LFB staining examined the extent of demyelinating (magnification: 200×).