Fig. 2.

Effect of CEACAM1 overexpression on VM-like network formation in HT1080 cells.

(A) Generation of CEACAM1-overexpressing HT1080 cells. Control (Luc) and CEACAM1-overexpressing HT1080 cells were cultured, and the cell lysates were immunoblotted with the indicated antibodies. (B and C) Inhibition of VM-like network formation by overexpression of CEACAM1 in HT1080 cells. Cells were seeded on Matrigel-coated wells, photographs were taken at 6 h after seeding (B), and the number of tubes was counted in 6 independent randomly selected fields (C). Scale bar, 100 μm. (D and E) Suppression of cell migration by overexpression of CEACAM1 in HT1080 cells. Cells were scratched and photographed under an inverted microscope at 0 and 12 h after the scratch. Photographs of 4 independent areas were taken; (D) the quantitative analysis of the relative area of migration is shown (E). Scale bar, 500 μm. (F) Suppression of cell growth by CEACAM1 in HT1080 cells. Luc and CEACAM1-overexpressing HT1080 cells were seeded at 1.2 × 10⁴ cells/well in 12-well plates and cultured for 3 h. Cells were recovered by trypsinization and counted using a hemocytometer every 24 h. Data shown are means ± SD. *P < 0.05.