Supplemental Figure 4.

The computational algorithm (ImmuCC) was used to predict the tissue composition of 25 immune cell types in untreated and treated wild-type (WT) and Marfan syndrome (MFS) lungs using microarray expression data. A and B: Graphs display the five most abundant cell types of the innate and adaptive immune system, respectively, visualized using CIBERSORT. Gray shading indicates mice treated with sildenafil. C: Representative images of hematoxylin and eosin–stained MFS and MFS SIL-treated lungs. Scale bars: 300 μm (C, main images); 200 μm (C, insets). NK, natural killer; PAP, predicted absolute proportion.