Table 2.

Average C_T & 18S rRNA average C_T measured, and ΔC_T, ΔΔC_T & fold difference calculated, for both Glut1&Glut4; in the N-diet, the HF-diet control group and the control diabetic group. The N-diet control group was considered as the reference control.

Groups	Glut1					Glut4
	Glut1 average CT	18S rRNA Average CT	ΔCT	ΔΔCT	2−ΔΔCt	Glut4 Average CT	18S rRNA Average CT	ΔCT	ΔΔCT	2−ΔΔCt
N-diet	31.22	28.16	3.06	0	1	36.28	35.43	0.85	0	1
HF-diet	30.33	24.97	5.36	2.30	0.20	33.64	30.13	3.51	2.66	0.16
Diabetic control	30.65	24.19	6.46	3.40	0.09	33.65	23.12	10.53	9.68	0.001

Where ΔC_T = Glut1 or Glut4 C_T- 18S rRNA C_T

ΔΔC_T = ΔC_T treated -ΔC_T nontreated(corresponding control).

2^−ΔΔCt = Fold difference in Glut 1 or Glut 4 relative to the nontreated control.