. 2019 Dec 2;27(2):689–694. doi: 10.1016/j.sjbs.2019.11.037

Table 3.

Average C_T & 18S rRNA average C_T measured, and ΔC_T, ΔΔC_T & fold difference calculated, for both Glut1&Glut4; in the HF-diet control group, nondiabetic control groups treated with curcumin, and the nontreated diabetic group. The HF-diet control group was considerd as the reference control.

Groups	Glut1					Glut4
Groups	Glut1 average C_T	18S rRNA average C_T	ΔC_T	ΔΔC_T	2^−ΔΔCt	Glut4 average C_T	18S rRNA average C_T	ΔC_T	ΔΔC_T	2^−ΔΔCt
Diabetic control	30.65	24.19	6.46	0	1	33.65	23.12	10.53	0	1
Curcumin	30.06	23.30	6.76	0.3	0.81	31.97	24.51	7.46	−3.07	8.40

Where ΔC_T = Glut1 or Glut4 C_T- 18S rRNA C_T

ΔΔC_T = ΔC_T treated -ΔC_T nontreated(corresponding control).

2^−ΔΔCt = Fold difference in Glut 1 or Glut 4 relative to the nontreated control.