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. 2020 Jan 17;23(2):100848. doi: 10.1016/j.isci.2020.100848

Figure 1.

Figure 1

Acidosis Stimulates Paracellular Gap Formation in Primary Vascular Endothelial Cells (ECs)

Plasma membrane staining and paracellular gap area quantitation of ECs treated for up to 5 h under physiological or acidic pH. Acidosis increases EC gap formation when compared with physiological pH treatment conditions.

(A) Representative pictures of plasma membrane staining in human umbilical vein endothelial cells (HUVECs) at 0, 3, and 5 h treated under physiological or acidic pH.

(B–E) Quantitative analysis of gap formation in (B) HUVECs, (C) human pulmonary artery endothelial cells (HPAECs), (D) human colon microvascular endothelial cells (HMVEC-Colon), and (E) human lung microvascular endothelial cells (HMVEC-Lung) over 5 h. All experiments were performed in triplicate and are representative of four experiments. Data at each time point are presented as mean ± SEM and analyzed for statistical significance between the pH 7.4 group and the pH 6.4 group using the unpaired t test where **p < 0.01 and ***p < 0.001. White arrows point to paracellular gaps. Scale bar, 100 μm.