Figure 10.

Immunohistochemistry of VCAM-1 and E-Selectin Protein Expression in the Loose Connective Dermal Tissue of Wild-Type Mice Treated with GPR4 Antagonist 13 or Vehicle

(A–J) Immunohistochemical analysis of adhesion molecules VCAM-1 and E-selectin protein expression in vascular endothelial cells within mouse dermal tissue sections of sham and cuff-affected hindlimbs. WT mice treated with GPR4 antagonist 13 had reduced expression of VCAM-1 and E-selectin in vascular endothelial cells in cuff-affected tissues when compared with vehicle control. Representative pictures of VCAM-1 signal in (A) WT vehicle sham, (B) WT vehicle cuff, (C) WT GPR4 antagonist 13 sham, and (D) WT GPR4 antagonist 13 cuff dermal connective tissues. Representative pictures of E-selectin expression can be visualized in (E) WT vehicle sham, (F) WT vehicle cuff, (G) WT GPR4 antagonist 13 sham, and (H) WT GPR4 antagonist 13 cuff dermal tissues. Quantitative scoring analysis of (I) VCAM-1 and (J) E-selectin protein expression in vascular endothelial cells in WT vehicle and WT GPR4 antagonist 13 cuff-affected dermal tissues. Pictures taken with 63x microscope objective. Scale bar indicates 100 μm. Arrows indicate blood vessels. Data are presented as mean ± SEM and were analyzed for statistical significance using the unpaired two-tailed t test where ***p < 0.001.