Figure 5.

GPR4 Deficiency Reduces Tissue Edema in the Inflammatory Hindlimb Ischemia-Reperfusion (IR) Mouse Model

(A and B) Upper and lower leg circumferences in wild-type (WT, N = 7) and GPR4 knockout (GPR4 KO, N = 6) mice were measured before and after IR. The change of leg circumference was calculated (value after IR – value before IR) and used as an indicator for tissue edema. GPR4 deficiency reduces the change of upper and lower leg circumference when compared with WT mice in the IR mouse model. Quantitative analysis of (A) upper and (B) lower leg circumference changes. Data are presented as mean ± SEM and analyzed for statistical significance using ANOVA where *p < 0.05, **p < 0.01, and ***p < 0.001.