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. 2020 Jan 17;23(2):100848. doi: 10.1016/j.isci.2020.100848

Figure 7.

Figure 7

Immunohistochemistry of VCAM-1 and E-Selectin Protein Expression in the Loose Connective Dermal Tissue of WT and GPR4 KO Mice

(A–J) Immunohistochemical analysis of adhesion molecules VCAM-1 and E-selectin protein expression in vascular endothelial cells within mouse dermal tissue sections of sham and cuff affected hindlimbs. GPR4-deficient mice had reduced expression of VCAM-1 and E-selectin in vascular endothelial cells in cuff-affected tissues when compared with WT. Representative pictures of VCAM-1 signal in (A) WT sham, (B) WT cuff, (C) GPR4 KO sham, and (D) GPR4 KO cuff tissues. Representative pictures of E-selectin expression can be visualized in (E) WT sham, (F) WT cuff, (G) GPR4 KO sham, and (H) GPR4 KO cuff tissues. Quantitative scoring analysis of (I) VCAM-1 and (J) E-selectin protein expression in vascular endothelial cells in WT and GPR4 KO cuff-affected dermal tissues. Pictures taken with 63x objective. Scale bar indicates 100 μm. Arrows indicate blood vessels. Data are presented as mean ± SEM and were analyzed for statistical significance using the unpaired two-tailed t test where ***p < 0.001.