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Effect of de-O-acetylation of Vi polysaccharide from C. freundii (A and B) and S. Typhi (C and D) on binding to human anti-Vi IgG 16/138 (A and C) and mouse monoclonal anti-Vi IgG (B, D) by PLL ELISA. Untreated (●), 0.05 M (△) and 1.5 M (○) ammonium hydroxide-treated Vi PS was co-coated with PLL to the plates. The results are the average of two assays and error bars represent standard deviations.
