Figure 1.
Pathogen-Induced SA Response in Roots, Revealed by the pPR1::eYFP-NLS Reporter
(A) SA contents in the roots of 5- or 10-day-old seedlings of Col-0, cpr6, and sid2 (sid2-3) measured by LC/MS-MS. n = 4 replicates, with multiple seedlings for each. Dots represent individual values, and lines indicate mean ± SD. Different letters represent significant difference; p < 0.05; by one-way ANOVA with a Tukey multiple comparison test.
(B–E) Induced pPR1::eYFP-NLS expression by P. syringae DC3000 (B and D) or SA (C and E) in roots.
(B and D) 5-day-old pPR1::eYFP-NLS seedlings were treated with P. syringae DC3000 (optical density 600 [OD600] = 0.01, ∼5 × 106 colony-forming units [CFUs]/mL) or with resuspension buffer (control) for 48 h and were then imaged by confocal laser scanning microscope (CLSM).
(C and E) For SA treatment, 5-day-old pPR1::eYFP-NLS seedlings were transferred to plates with DMSO or 40 μM SA for 24 h and were then imaged by CLSM. Scale bars, 10 μm. For quantification, the average GFP florescence of 5–10 representative cells from 10 seedlings for each treatment was measured by Fiji. The data points were shown as dot plots. Dots represent individual values, and lines indicate mean ± SD. p values were calculated by a two-tailed t test.
See also Figure S1.