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. 2020 Feb 3;30(3):381–395.e8. doi: 10.1016/j.cub.2019.11.058
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Rabbit anti-PIN1 [37] N/A
Rabbit anti-PIN2 [36] N/A
Mouse anti-His-tag monoclonal Antibody Agrisera Cat# AS11 1771
Mouse anti-myc-tag monoclonal Antibody, clone 4A6 Millipore (Merck) Cat# 05-724
Phospho-PP2A alpha (Tyr307) Polyclonal Antibody Thermo Scientific Cat# PA5-36874; RRID:AB_2553794
Anti-GFP-HRP Miltenyi Biotec Cat# 130-091-833; RRID:AB_247003
Monoclonal anti-GFP antibody produced in mouse Sigma Cat# G6539; RRID:AB_259941
Anti-PIP2;1 [65] N/A

Bacterial and Virus Strains

Escherichia coli DH5α Lab stock N/A
E.coli BL21 (DE3) New England Biolabs Cat# C2527H
Agrobacterium tumefaciens GV3101 Lab stock N/A
Pseudomonas syringae pv. tomato DC3000 Armin Djamei lab N/A

Chemicals, Peptides, and Recombinant Proteins

brefeldin A Sigma Cat# B7651
Propidium Iodide Sigma Cat# P3566
PBS Buffer 10 × (1000 mL) GE Healthcare Cat# BR100672
PhosSTOP Sigma/Roche Cat# 4906837001
cOmplete protease inhibitor cocktail Sigma/Roche Cat# 4693124001
Benzoic acid (BA) Sigma Cat# 242381
Salicylic Acid (SA) Sigma Cat# 247588
3-Hydroxybenzoic acid (3-OH-BA) Sigma Cat# H20008
4-Hydroxybenzoic acid (4-OH-BA) Sigma Cat# H20059
N-(1-Naphthyl)phthalamidic acid Sigma Cat# N12507
2,3,5-Triiodobenzoic acid (TIBA) Sigma Cat# T5910
[3H]-IAA (([5-3H]-Indole-3-acetic acid) American Radiolabeled Chemicals Cat# ART 0340
[3H]- NAA ([4-3H]-1-Naphthylacetic acid) American Radiolabeled Chemicals Cat# ART 0610
[3H]-2,4-D (([5-3H]-2,4-Dichlorophenoxy acetic acid) American Radiolabeled Chemicals Cat# ART 0559
Cantharidin Sigma Cat# C7632
Imidazole Sigma Cat# I5513
FastDigest Hin1II Thermo Fisher Scientific Cat# FD1834
FastDigest EcoRI Thermo Fisher Scientific Cat# FD0274
FastDigest XhoI Thermo Fisher Scientific Cat# FD0694
FastDigest BamHI Thermo Fisher Scientific Cat# FD0054
FastDigest SalI Thermo Fisher Scientific Cat# FD0644
T4 DNA Ligase Buffer Thermo Fisher Scientific Cat# 46300-018
T4 DNA Ligase (1 U/μL) Thermo Fisher Scientific Cat# 15224-017
GeneJET Plasmid Miniprep Kit Thermo Fisher Scientific Cat# K0503
GeneJET Gel extraction kit Thermo Fisher Scientific Cat# K0692
BSA (Bovine Serum Albumin) Sigma Cat# A2153
His-PP2AA1 This study N/A
His-PP2AA3 This study N/A
His-PIN2HL This study N/A

Critical Commercial Assays

Bio-Safe Coomassie Stain #1610786 Bio-Rad Cat# 1610786
Non-Radioactive Phosphatase Assay Systems Promega Cat# V2460
HisPur Ni-NTA Resin Thermo Fisher Scientific Cat# 88222
γ-[32P]-ATP PerkinElmer Cat# NEG502A001MC

Experimental Models: Cell Lines

Nicotiana tabacum L., cv. Bright Yellow-2 (BY-2) N/A N/A

Experimental Models: Organisms/Strains

Arabidopsis thaliana Col-0 N/A N/A
A. thaliana Ws-4 NASC N5390
A. thaliana eir1-4 (pin2-T) [36] SALK_091142
A. thaliana aux1-T (aux1) [66] SALK_020355
A. thaliana pAUX1::AUX1-YFP [67] N/A
A. thaliana pPR1::eYFP-NLS [24] N/A
A. thaliana npr1-1 [2] N/A
A. thaliana npr3-1 npr4-3 [68] N/A
A. thaliana npr1-1 npr3-1 npr4-3 [68] N/A
A. thaliana cpr6 [23] N/A
A. thaliana sid2-3 [69] SALK_042603
A. thaliana rcn1-1 (rcn1, pp2aa1-1) [42] N/A
A. thaliana rcn1-6 (pp2aa1-6) [49] SALK_059903
A. thaliana pp2aa2-2 [45] SALK_037095
A. thaliana pp2aa2-3 [45] SALK_017541
A. thaliana pp2aa3-2 [45] SALK_099550
A. thaliana pp2aa1 pp2aa2-3 [45] N/A
A. thaliana pp2aa1 pp2aa3-1 [45] N/A
A. thaliana pPIN2::PIN2-GFP [70] N/A
A. thaliana pPP2AA1::PP2AA1-GFP [45] N/A
A. thaliana DR5v2 [28] N/A
A. thaliana eir1-4 DR5v2 This study N/A
A. thaliana pPR1::eYFP-NLS [24] N/A
A. thaliana pp2aa1-6 pPR1::eYFP-NLS This study N/A
A. thaliana 35S::4 × myc-PP2AA1 This study N/A
A. thaliana pp2aa1-6 cpr6 This study N/A

Oligonucleotides

Primers used in this study, see Table S1 This study N/A

Recombinant DNA

Plasmid pET28a-PP2AA1 This study N/A
Plasmid pET28a-PP2AA3 This study N/A
Plasmid pET28a-PIN2HL This study N/A
Plasmid pEGAD-35S::4 × myc-PP2AA1 This study N/A

Software and Algorithms

Arabidopsis Information Resource (TAIR) http://www.arabidopsis.org/ N/A
ImageJ https://imagej.nih.gov/ij/ NIH
Fiji https://fiji.sc/ N/A
ZEN https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html ZEISS
DNA MAN https://www.lynnon.com/ N/A
ChemSketch https://www.acdlabs.com/resources/freeware/chemsketch/ N/A