Antibodies |
|
Rabbit anti-PIN1 |
[37] |
N/A |
Rabbit anti-PIN2 |
[36] |
N/A |
Mouse anti-His-tag monoclonal Antibody |
Agrisera |
Cat# AS11 1771 |
Mouse anti-myc-tag monoclonal Antibody, clone 4A6 |
Millipore (Merck) |
Cat# 05-724 |
Phospho-PP2A alpha (Tyr307) Polyclonal Antibody |
Thermo Scientific |
Cat# PA5-36874; RRID:AB_2553794
|
Anti-GFP-HRP |
Miltenyi Biotec |
Cat# 130-091-833; RRID:AB_247003
|
Monoclonal anti-GFP antibody produced in mouse |
Sigma |
Cat# G6539; RRID:AB_259941
|
Anti-PIP2;1 |
[65] |
N/A |
|
Bacterial and Virus Strains |
|
Escherichia coli DH5α |
Lab stock |
N/A |
E.coli BL21 (DE3) |
New England Biolabs |
Cat# C2527H |
Agrobacterium tumefaciens GV3101 |
Lab stock |
N/A |
Pseudomonas syringae pv. tomato DC3000 |
Armin Djamei lab |
N/A |
|
Chemicals, Peptides, and Recombinant Proteins |
|
brefeldin A |
Sigma |
Cat# B7651 |
Propidium Iodide |
Sigma |
Cat# P3566 |
PBS Buffer 10 × (1000 mL) |
GE Healthcare |
Cat# BR100672 |
PhosSTOP |
Sigma/Roche |
Cat# 4906837001 |
cOmplete protease inhibitor cocktail |
Sigma/Roche |
Cat# 4693124001 |
Benzoic acid (BA) |
Sigma |
Cat# 242381 |
Salicylic Acid (SA) |
Sigma |
Cat# 247588 |
3-Hydroxybenzoic acid (3-OH-BA) |
Sigma |
Cat# H20008
|
4-Hydroxybenzoic acid (4-OH-BA) |
Sigma |
Cat# H20059
|
N-(1-Naphthyl)phthalamidic acid |
Sigma |
Cat# N12507 |
2,3,5-Triiodobenzoic acid (TIBA) |
Sigma |
Cat# T5910 |
[3H]-IAA (([5-3H]-Indole-3-acetic acid) |
American Radiolabeled Chemicals |
Cat# ART 0340 |
[3H]- NAA ([4-3H]-1-Naphthylacetic acid) |
American Radiolabeled Chemicals |
Cat# ART 0610 |
[3H]-2,4-D (([5-3H]-2,4-Dichlorophenoxy acetic acid) |
American Radiolabeled Chemicals |
Cat# ART 0559 |
Cantharidin |
Sigma |
Cat# C7632 |
Imidazole |
Sigma |
Cat# I5513 |
FastDigest Hin1II |
Thermo Fisher Scientific |
Cat# FD1834 |
FastDigest EcoRI |
Thermo Fisher Scientific |
Cat# FD0274 |
FastDigest XhoI |
Thermo Fisher Scientific |
Cat# FD0694 |
FastDigest BamHI |
Thermo Fisher Scientific |
Cat# FD0054 |
FastDigest SalI |
Thermo Fisher Scientific |
Cat# FD0644 |
T4 DNA Ligase Buffer |
Thermo Fisher Scientific |
Cat# 46300-018 |
T4 DNA Ligase (1 U/μL) |
Thermo Fisher Scientific |
Cat# 15224-017 |
GeneJET Plasmid Miniprep Kit |
Thermo Fisher Scientific |
Cat# K0503 |
GeneJET Gel extraction kit |
Thermo Fisher Scientific |
Cat# K0692 |
BSA (Bovine Serum Albumin) |
Sigma |
Cat# A2153 |
His-PP2AA1 |
This study |
N/A |
His-PP2AA3 |
This study |
N/A |
His-PIN2HL |
This study |
N/A |
|
Critical Commercial Assays |
|
Bio-Safe Coomassie Stain #1610786 |
Bio-Rad |
Cat# 1610786 |
Non-Radioactive Phosphatase Assay Systems |
Promega |
Cat# V2460 |
HisPur Ni-NTA Resin |
Thermo Fisher Scientific |
Cat# 88222 |
γ-[32P]-ATP |
PerkinElmer |
Cat# NEG502A001MC |
|
Experimental Models: Cell Lines |
|
Nicotiana tabacum L., cv. Bright Yellow-2 (BY-2) |
N/A |
N/A |
|
Experimental Models: Organisms/Strains |
|
Arabidopsis thaliana Col-0 |
N/A |
N/A |
A. thaliana Ws-4 |
NASC |
N5390 |
A. thaliana eir1-4 (pin2-T) |
[36] |
SALK_091142 |
A. thaliana aux1-T (aux1) |
[66] |
SALK_020355 |
A. thaliana pAUX1::AUX1-YFP |
[67] |
N/A |
A. thaliana pPR1::eYFP-NLS |
[24] |
N/A |
A. thaliana npr1-1 |
[2] |
N/A |
A. thaliana npr3-1 npr4-3 |
[68] |
N/A |
A. thaliana npr1-1 npr3-1 npr4-3 |
[68] |
N/A |
A. thaliana cpr6 |
[23] |
N/A |
A. thaliana sid2-3 |
[69] |
SALK_042603 |
A. thaliana rcn1-1 (rcn1, pp2aa1-1) |
[42] |
N/A |
A. thaliana rcn1-6 (pp2aa1-6) |
[49] |
SALK_059903 |
A. thaliana pp2aa2-2 |
[45] |
SALK_037095 |
A. thaliana pp2aa2-3 |
[45] |
SALK_017541 |
A. thaliana pp2aa3-2 |
[45] |
SALK_099550 |
A. thaliana pp2aa1 pp2aa2-3 |
[45] |
N/A |
A. thaliana pp2aa1 pp2aa3-1 |
[45] |
N/A |
A. thaliana pPIN2::PIN2-GFP |
[70] |
N/A |
A. thaliana pPP2AA1::PP2AA1-GFP |
[45] |
N/A |
A. thaliana DR5v2 |
[28] |
N/A |
A. thaliana eir1-4 DR5v2 |
This study |
N/A |
A. thaliana pPR1::eYFP-NLS |
[24] |
N/A |
A. thaliana pp2aa1-6 pPR1::eYFP-NLS |
This study |
N/A |
A. thaliana 35S::4 × myc-PP2AA1 |
This study |
N/A |
A. thaliana pp2aa1-6 cpr6 |
This study |
N/A |
|
Oligonucleotides |
|
Primers used in this study, see Table S1
|
This study |
N/A |
|
Recombinant DNA |
|
Plasmid pET28a-PP2AA1 |
This study |
N/A |
Plasmid pET28a-PP2AA3 |
This study |
N/A |
Plasmid pET28a-PIN2HL |
This study |
N/A |
Plasmid pEGAD-35S::4 × myc-PP2AA1 |
This study |
N/A |
|
Software and Algorithms |
|
Arabidopsis Information Resource (TAIR) |
http://www.arabidopsis.org/ |
N/A |
ImageJ |
https://imagej.nih.gov/ij/ |
NIH |
Fiji |
https://fiji.sc/ |
N/A |
ZEN |
https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html |
ZEISS |
DNA MAN |
https://www.lynnon.com/ |
N/A |
ChemSketch |
https://www.acdlabs.com/resources/freeware/chemsketch/ |
N/A |