Skip to main content
. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Ticks Tick Borne Dis. 2019 Dec 6;11(2):101354. doi: 10.1016/j.ttbdis.2019.101354

Figure 4. OmCI reduces the ability of serum from domestic and wild avian hosts to eradicate a serum sensitive B. burgdorferi strain.

Figure 4.

A low passage, infectious, and serum resistant B. burgdorferi strain B31-5A4 (“B31-5A4”) or a high passage, a non-infectious, and serum sensitive B. burgdorferi strain B313 (“B313”) was incubated for 4h with the serum from (A) Coturnix quail (“quail”), (B) chicken, (C) geese, (D) turkey, (E) American robins (“robins”), or (F) Gray catbirds (“catbirds”) at a final concentration of 40 % in the presence (“OmCI-serum”) or absence (“serum”) of 2 μM of OmCI. Note that the results from quail were derived from Figure 3. The heat-inactivated serum from the above-mentioned animals was included as a control (“heat-treated”). The number of motile spirochetes was assessed microscopically. The percentage of survival for those B. burgdorferi strains was calculated using the number of mobile spirochetes at 4 h post incubation normalized to that prior to the incubation with serum. The experiments were performed on three independent experiments; within each experiment, samples were run in triplicate, and the survive percentage for each experiment was calculated by averaging the results from triplicate experiments. The result shown here is the average ± standard deviation of the survival percentage from three independent experiments. (*), the significant difference (P < 0.05) of the percent survival of spirochetes between indicated groups was determined using the one-way ANOVA with post hoc Dunn’s test.