Fig. 2.

Construction of genome-edited strains expressing 5′-UTR–engineered enzymes of the pathway. Cell growth and production of ent-kaurene in batch bioreactor fermentation are presented too. a Schemes of construction of strains MGIG, MGIUG, and MGIUK. b Redesign of the 5′-UTRs of GGPPS, CDPS, KS, trKO, and trCYP714A2. c Cell growth of strains MGIUK and MGI/GGPPS_CDPS_KS in batch fermentation with 20 g/L glycerol as a carbon source. dEnt-kaurene production by strains MGIUK and MGI/GGPPS_CDPS_KS in batch fermentation. The results represent means from three independent experiments