Fig. 5.

PTEN is targeted and negatively regulated by miR-29b-3p, and the silencing of PTEN attenuates the OGD-induced injury on neurons and BMECs. a The binding sites between miR-29b-3p and PTEN gene predicted by TargetScan website. b The regulation of miR-29b-3p on PTEN gene transcription verified by dual luciferase reporter gene assay. c mRNA level of PTEN was detected by RT-qPCR in neurons and BMECs infected with lentivirus harboring miR-29b-3p. d The protein expression of PTEN detected by western blot analysis in neurons and BMECs infected with lentivirus harboring miR-29b-3p. The mRNA e and protein f expression of PTEN were elevated in MCAO rats and OGD cells. g, h Western blot analysis of neuronal apoptosis- and BMECs angiogenesis-related proteins in neurons and BMECs infected with lentivirus harboring miR-29b-3p and or PTEN shRNA. i The tube formation ability of BMECs infected with lentivirus harboring miR-29b-3p and or PTEN shRNA (scale bar = 100 μm). *p < 0.05 vs. the PGL-3 vector group, NC group, sham group, control group or Lv-vector group. #p < 0.05 vs. the Lv-miR-29b-3p group or Lv-sh-PTEN group. Lv Lentivirus. The measurement data are expressed as mean ± standard deviation. The comparison between the two groups was carried out by the independent sample t test. Comparison among multiple groups was conducted using one-way analysis of variance, followed by Tukey’s post hoc test. The experiment was repeated three times