Fig. 5.

RhoC depletion rescues the ARHGAP18 mediated effects on YAP activity. a Overexpression of wild type ARHGAP18 (WTGAP18) or mutant ARHGAP18 (M-GAP18) in ECs and quantification of expression of pYAP, CyR61 and p65. Representative images of one experiment is shown and quantification of the proteins from n = 3 experiments. p by paired t-test. Levels of ARHGAP18 and pYAP were calculated relative to actin. Levels of CyR61 and p65 were calculated relative to levels of ARHGAP18. b Cells were treated with siRNA to ARHGAP18, then 24 h later treated with siRNA to RhoC and 48 h later, lysates were made and probed for indicated proteins. Representative images of one experiment is shown and quantification of the proteins from n = 3–6 experiments is given. c Cells were treated with siRNA to ARHGAP18 or RhoC and probed for the indicated proteins. Western from a representative experiment to indicate that RhoC knockdown alone was effective on RhoC and had no significant effect on ARHGAP18, YAP, CyR61 expression