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. 2020 Feb 3;16:10. doi: 10.1186/s13007-020-0556-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — The consistency and reproducibility of our N-ChIP protocol validated by ChIP-seq profiles. a A venn diagram showing the overlap between the H3K9/K14ac-enriched peaks called by ChIP-seq replicate 1 and 2. b IGV browser shots illustrating chromosome-wide and local H3K9/K14ac enrichments for the two biological replicates. c, d Metagene analysis illustrating a positive correlation between local H3K9/K14ac enrichment and gene expression levels. The expressed genes were evenly classified into five groups according to their transcript levels (blue-highest expression, light blue-high, green-medium, yellow-low and red-lowest). The group of genes with no detectable transcripts was included as well (black line). The transcriptome data were published previously [30]