Fig. 1.
Growth of four yeast strains in the Duetz-MTPS system using 10 different media. The graphs show CDW (g/L) after 24 h cultivation (values are mean ± SD; n = 3), whereas Additional file 1: Figs. S1 and S2 provide more complete growth curves and pH profiles. Conditions: Glucose, 50 g/L; Kjeldahl nitrogen, 5.86 g/L; ODinitial = 0.5; volume: 2.5 mL; pHinitial= 5.0; incubation at 30 °C with 450 rpm shaking. The pH and pO2 were not controlled and several of the apparent differences between media may be due to buffering effects. YNBAS yeast nitrogen base without amino acids and with ammonium sulfate, YNBU yeast nitrogen base without amino acids and with urea, YP yeast extract and meat peptone, CH chicken by-products hydrolysate, B BALI™ hydrolysate, G glucose