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. 2020 Feb 3;51:8. doi: 10.1186/s13567-020-0736-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Gga-miR-200a-3p repressed the expression of the immune target genes related to MAPK signaling pathway. The expression profile of gga-miR-200a-3p A its candidate target genes. B–D The signaling molecules of MAPK signaling pathway E–G and Western blot analysis of the signaling molecules H in the HD11 chicken macrophage cell line treated with mimic miRNA and LPS.Expression data were transformed to a relative fold expression value in arbitrary units (AU) using the control expression level as a calibrator. Results are expressed as the mean ± SEM (n = 3) of three independent experiment.