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. 2020 Jan 10;14(2):309–328. doi: 10.1002/1878-0261.12622

Figure 9.

Figure 9

SOCS3 and EZH2 essentially mediated the effects of DANCR on inflammation. (A) qRT‐PCR showed that overexpression of SOCS3 or inhibition of EZH2 with GSK‐126 reduced DANCR‐stimulated mRNA of IL‐6 of indicated cells. (B) qRT‐PCR showed that overexpression of SOCS3 or inhibition of EZH2 with GSK‐126 reduced DANCR‐stimulated mRNA of TGF‐β of indicated cells. (C,D) Western blot showed that SOCS3 and GSK‐126 antagonized the effect of DANCR in activating p65 and STAT3. (E,F) Western blot on H3K27me3, H3, and SOCS3 showed that although overexpression of SOCS3 failed to alter the level of H3K27me3, whereas GSK‐126 significantly reduced it, both treatments significantly boosted the level of SOCS3 in DANCR cells. One‐way ANOVA was used to determine statistical significance: *P < 0.01, **P < 0.05.