Figure 5.

ECE1c^K6R expression promotes EMT‐linked invasiveness. (A) DLD‐1 clone cells expressing either Flag‐tagged ECE1c^WT or ECE1c^K6R proteins (or Mock cells) were grown for 22 h in matrigel chambers. Invasive capability was determined by counting crystal violet‐stained cells (upper panel: representative images; scale bar = 50 µm). Relative quantification of stained cells is shown (lower panel). (B) As in A, cells were grown under normal conditions for 48 h, and the mRNA levels of E‐cadherin, N‐cadherin, and Snail were determined using RT‐qPCR. (C) Protein levels of E‐cadherin, N‐cadherin, and Snail from cells in B were determined using western blot. Data represent average ± SEM (n = 3). ANOVA and Tukey tests were used. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.