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. 2020 Jan 10;14(2):387–406. doi: 10.1002/1878-0261.12626

Figure 3.

Figure 3

SNX16 promotes CRC cell proliferation by activating the c‐Myc signaling pathway. (A, B) GSEA demonstrated that MYC was positively associated with a high‐SNX16‐expression CRC group (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17536, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE40967, http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE32323, and http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE44861). NES, normalized enrichment score; FDR, false discovery rate. (C, D) The expression of SNX16 and c‐Myc in SNX16‐knockdown or SNX16‐overexpressing cells was measured by qRT‐PCR and western blotting. (E) Western blots of the indicated proteins in SW480 cells (LV‐NC vs. LV‐SNX16) treated with the c‐Myc inhibitor 10058‐F4. (F) Western blots of indicated proteins in HT29 cells (sh‐NC vs. sh‐SNX16) with/without ectopic expression of c‐Myc. (G) MTT assay. The results are shown as the means ± SEMs (n = 5), ****P < 0.0001. (H) Colony formation assays. The results are shown as the means ± SEMs (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.