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. 2020 Feb 3;13:12. doi: 10.1186/s13048-020-0615-0

Fig. 4.

Fig. 4

Age-dependent DNA methylation of autophagy-related genes in the ovaries of rats. (a) Agarose gel electrophoresis of DNA methylation. (b-c) Quantitative methylation levels of Atg5 and Lc3B. .Atg5 gene and Lc3B gene methylation levels were significantly higher in the ovaries of 24 M rats than in those of 6 M rat. 6 M: 6-month-old, 12 M: 12-month-old, 24 M: 24-month-old, M: methylated bands, U: unmethylated bands. Compared with 6 M rats, *P < 0.05, ***P < 0.001; compared with 12 M rats, ###P < 0.001