FIGURE 1.

Phenotypic screening of small molecule library obtained from NCI-DTP. (A) The small molecule library was evaluated for antimycobacterial activity at a single concentration of 10 μM. Bars are showing number of hits that possessed activity >10 μM, between 5 and 10 μM, between 5 and 2.5 μM, and less than 2.5 μM. (B) MIC₉₉ values for the few selected small molecules were determined as mentioned in section “Materials and Methods.” The value on y-axis denotes MIC₉₉ values and x-axis denotes Ids of compounds tested for antimycobacterial activity. (C) Chemical structure of most potent hit (NSC 18725; 3,5-dimethyl-4-nitroso-1-phenylpyrazole) identified in our phenotypic screening.