TLC analysis of the “crude” “Folch” extract before (lane 3) and after washing with saturated KCl solution (lane 2) of the cellular lipid by Mortierella isabellina ATHUM 2935, cultivated on glucose. Lane 1: mix of neutral lipid standards (cholesterol‐CL, oleic acid‐FFA, glyceryl trioleate‐TAG, cholesteryl linoleate, CE). Analysis was performed when lipid in DCW presented its highest value.