Table 2.
Overview of interferences of cell integrity assays with permeabilization agents
| Assay | Control DeLisa | Tris 200 mM | Tris 300 mM | Tris 400 mM | Tris 500 mM | Guanidine 200 mM | Triton 0.5 wt% | Urea 2 M | BaCl 60 mg/L | BaCl 300 mg/L |
|---|---|---|---|---|---|---|---|---|---|---|
| Nanodrop (DNA) | − | − | − | − | − | − | − | − | − | − |
| PicoGreen (DNA) | + | + | + | + | + | n/a | n/a | n/a | n/a | n/a |
| CP marker (β‐Gal) | + | +/red | +/red | +/red | +/red | + | +/inc | + | − | + |
| PP marker (AP) | +/red | +/red | +/red | +/red | +/red | +/red | +/red | +/red | +/red | +/red |
| PP product (HRP) | + | +/red | +/red | +/red | +/red | + | + | +/inc | +/inc | +/inc |
| Total protein (bicinchoninic acid) | − | − | − | − | − | − | − | − | − | − |
| Total protein (Bradford) | + | +/inc | +/inc | +/inc | +/inc | + | − | + | − | +/inc |
+, no interference; −, strong interference; red, slightly reduced signal; inc, slightly increased signal; n/a, not available; BaCl, benzalkonium chloride.