Table 6.
Impact of preparation parameters on physico‐chemical media characteristics (F‐media). The coefficients in terms of coded factors of the DoE are presented for F‐media. Confidence interval was set to 95% for all responses. Interaction effects are denoted as A × C
| (A) | (B) | (C) | |||||
|---|---|---|---|---|---|---|---|
| F‐media | Range | Temp. (30–37°C) | P/V (64–110 W/m3) | Prep. time (110–170 min.) | A × C | R 2 | Q 2 |
| Osmolality | 1276–1341 [mOsm/kg] | – | – | – | – | – | – |
| pH | 6.93–6.97 [‐] | – | – | – | – | – | – |
| pCO2 | 1–4 [%] | −0.74 | −0.53 | −0.69 | – | 0.93 | 0.88 |
| ORP | −227–−253 [mV] | −9.44 | 0.84 | −4.37 | – | 0.99 | 0.98 |
| DO average | 10–23 [%] | −2.2 | 3.51 | – | – | 0.89 | 0.85 |
| 2D‐FL (ex450/em530) | 1215–1363 [IU] | 30.78 | – | 50.14 | 12 | 0.95 | 0.92 |
| 2D‐FL (ex290/em370) | 1242–1439 [IU] | −83.9 | – | 9.30 | – | 0.97 | 0.95 |
DO, dissolved oxygen; F‐media, feed media ORP, oxidation reduction potential; P‐media, production/basal media; P/V, specific power input; Q 2, goodness of prediction; R 2, goodness of fit.