Table 7.
Impact of preparation parameters and storage time on process performance. The coefficients in terms of coded factors of the DoE are presented for P‐ and F‐media. Confidence interval was set to 95% for all responses. Values in italic are not significant. Interaction effects are denoted as B × C
| (A) | (B) | (C) | (D) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Range | Temp. (28–38°C) | P/V (30–130 W/m3) | Prep. Time (65–160 min) | Storage time (4–18 days) | B × C | D 2 | R² | Q² | |
| P‐media | |||||||||
| qlac (cell line B) | 1.57–2.37 [pmol/cell/day] | – | – | – | 0.29 | – | – | 0.60 | 0.48 |
| Product titer (cell line A) | 2.6–2.8 [g/l] | – | 0.016 | – | −0.086 | – | – | 0.59 | 0.40 |
| µaverage(cell line A) | 0.29–0.41 [1/d] | – | −0.017 | 0.096 | −0.034 | −0.012 | – | 0.71 | 0.44 |
| F‐media | |||||||||
| Harvest viability (cell line B) | 79–88 [%] | 0.066 | −1.74 | −1.57 | 0.45 | – | 3.26 | 0.78 | 0.40 |
F‐media, feed media; P‐media, production/basal media; P/V, specific power input; Q 2, goodness of prediction; R 2, goodness of fit.