Figure 3.

Product formation of dihydrocarvone at different reactions for a mediated cofactor substitution or regeneration (reaction conditions: 5 mM R‐(−)‐carvone, experiments with Cp*Rh(bpy) were performed in TRIS buffer (100 mM, pH 7, 10 mM CaCl₂), all other experiments in MOPS buffer (50 mM, pH 7, 10 mM CaCl₂), Cp*Rh(bpy), CoSep, and Safranin T: 250 μM mediator; Cp*Rh(bpy) + FMN: 100 μM mediator and 100 μM FMN; Cp*Rh(bpy) + NADP⁺: 50 μM mediator and 100 μM NADP⁺ (n = 2). An additional experiment with safranine T as mediator in the Tris/HCl buffer was performed. Again, no product concentration could be measured. Therefore it can be concluded that the buffer has no significant influence on the results.