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The aptamer‐dissociation from apta‐beads complex in response to spiked OTA in beer. The decrease in Ct value in response to higher OTA concentrations reflects to the dissociation of aptamer from apta‐beads complex into the supernatant. The assay was able to detect OTA with the linear relationship of Ct values from 0.1 to 1000 ng/mL, R 2 = 0.95. The LOD of the Apta‐qPCR was found to be 0.078 ng/mL OTA as shown in inset figure.
