Figure 2.

Morphological characteristics of yeasts LBs and ascospores during the lipogenic phase (72–96 h). Left panels: Transmitted light micrographs (1000x), right panels: fluorescence images of LBs and ascospores (AS) labeled with Nile red. Group I: (A) Candida boidinii EL1; (B) Candida ethanolica M1; (C) Candida metapsilosis EL2; (D) Candida parapsilosis LV2; (E) Candida pararugosa BM24; Group II: (F) Pichia sp. M3; (G) Pichia sp. M31; (H) Pichia manshurica EP2; (I) Pichia membranifaciens O2; (J) Pichia membranifaciens LC1R; (K) Pichia manshurica V6; (L) Pichia etchellsii BM1; (M) Pichia anomala EC28; Group III: (N) Kluyveromyces marxianus CC1. Culture conditions: growth on 250‐mL flasks in 50 mL of NLM; agitation rate 180 rpm; temperature 28 ± 1°C; initial glucose concentration 30 g L⁻¹; initial pH 6 ± 0.1.