Table 1.
Plasmids, oligonucleotides, and DNA fragments used in this study
| Plasmids, primers, or DNA fragments | Description, sequence, or primers/template | Note |
|---|---|---|
| pDG1662 | B. subtilis integration vector, amyE locus, ampR and specR in E. coli, cmR in B. subtilis | Guerout‐Fleury, AM et.al. 8973347 |
| pBS1 | pDG1662 derivative, cotC‐petase | This work |
| pBS2 | pDG1662 derivative, cotG‐mhetase | This work |
| pBS3 | pDG1662 derivative, cotC‐petase, cotG‐mhestase | This work |
| 1178R | Ggttagtgacattagaaaaccgactg | This work |
| 1219F | Cagtcggttttctaatgtcactaacctgtaggataaatcgtttgggcc | This work |
| 1221F | Cagtcggttttctaatgtcactaaccagtgtccctagctccgagaaaaaatcc | This work |
| 1224F | Tcgacatggatgagcgatgatgatatccg | This work |
| 1230F | Aattctccagtcttcacatcggtttg | This work |
| 1233R | Gcaaatgcagacaatatcagcatcc | This work |
| 1342F | Tcttgagtccaacccggtaagacac | This work |
| 1343R | Cgatagttaccggataaggcgcag | This work |
| 1476R | cggatatcatcatcgctcatccatgtcgattaatgatggtggtggtggtgagagcag | This work |
| 1478R | Cggatatcatcatcgctcatccatgtcgattaaggaggagcagcgcaagcg | This work |
| 1479R | Ctttgagtgatctgataccgctcg | This work |
| 1480F | Cgagcggtatcagctcactcaaagtgtaggataaatcgtttgggcc | This work |
| 1629F | Cagtcggttttctaatgtcactaaccgactgcaacgggcaatatgtctctg | This work |
| 1630R | Ggcccaaacgatttatcctacagcatattgatccgccactgcctgg | This work |
| 1631F | Tgtaggataaatcgtttgggcc | This work |
| 1632R | Ggcccaaacgatttatcctacaaaccgacatcgctttcaacattg | This work |
| 1633R | Ggcccaaacgatttatcctacagagatatcatcaccaacaagctg | This work |
| 1634R | Ggcccaaacgatttatcctacatcatcgcgaccggcaataagagg | This work |
| 1635R | Ggcccaaacgatttatcctacactgcggtgccagcgcaatctatctg | This work |
| PCR1 | 1342F, 1178R, pDG1662, 5′ amyE + camR | This work |
| PCR2 | 1224F, 1343R, pDG1662, 3′ amyE | This work |
| PCR3 | 1224F, 1178R, pDG1662 backbone | This work |
| PCR4 | 1230F, 1178R, pDG1662, 5′ amyE + camR | This work |
| PCR5 | 1224F, 1233R, pDG1662, 3′ amyE | This work |
| PCR6 | 1219F, 1476R, synthesized DNA, cotC‐petase | This work |
| PCR7 | 1221F, 1478R, synthesized DNA, cotG‐mhetase | This work |
| PCR8 | 1221F, 1479R, synthesized DNA, cotC‐petase | This work |
| PCR9 | 1480F, 1476R, synthesized DNA, cotG‐mhetase | This work |
| PCR10 | 1631F, 1343R, assembled linear DNA 3, cotC‐petase + 3′ amyE | This work |
| PCR11 | 1629F, 1630R, E. coli chromosome | This work |
| PCR12 | 1629F, 1632R, E. coli chromosome | This work |
| PCR13 | 1629F, 1633R, E. coli chromosome | This work |
| PCR14 | 1629F, 1634R, E. coli chromosome | This work |
| PCR15 | 1629F, 1635R, E. coli chromosome | This work |
| DNA 1 | PCR4 + PCR6 + PCR5, 4 kb | This work |
| DNA 2 | PCR4 + PCR7 + PCR5, 6 kb | This work |
| DNA 3 | PCR1 + PCR6 + PCR2, 8 kb | This work |
| DNA 4 | PCR1 + PCR8 + PCR9 + PCR2, 10 kb | This work |
| DNA 5 | PCR1 + PCR12 + PCR10, 12 kb | This work |
| DNA 6 | PCR1 + PCR13 + PCR10, 14 kb | This work |
| DNA 7 | PCR1 + PCR14 + PCR10, 16 kb | This work |
| DNA 8 | PCR1 + PCR15 + PCR10, 18 kb | This work |