Figure 3.
Effect of methanol concentration on partitioning and yield of proteins. The optimization of the methanol/tripotassium phosphate system was performed by varying the methanol concentration from 20–40% (w/w), while the concentration of K3PO4 was maintained at 30% (w/w). Above methanol concentration of 40% (w/w), white insoluble complex was formed upon the addition of BCA working reagent into the sample and blocked absorbance measurement. Partition coefficient and yield were calculated using Eqs. (1), and (2), (3), respectively. The results were expressed as the means of triplicate readings (mean ± SD).