Table 3.
Quantitative data of Rhodosporidium toruloides DSM 4444 originated from kinetics in shake‐flask experiments in sterilized and pasteurized media, supplemented with 4.0% w/v NaCl and 50 and 100 g/L initial glucose concentration (A) and from kinetics in media containing 50, 100, and 150 g/L of glucose and 4% w/v NaCl in batch‐bioreactor experiments (B)
| A) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Culture mode and heat‐treatment | Glci (g/L) | Time (h) | Glccons (g/L) | X (g/L) | L (g/L) | YL/X (%,w/w) | YL/Glc (g/g) | YX/Glc (g/g) |
| Flasks, sterilized | ≈50 | 192 | 48.6 | 9.4 | 6.7 | 71.3 | 0.14 | 0.19 |
| Flasks, pasteurized | ≈50 | 160 | 48.0 | 11.7 | 5.9 | 50.4 | 0.12 | 0.24 |
| Flasks, sterilized | ≈100 | 505 | 93.0 | 16.1 | 9.2 | 57.1 | 0.10 | 0.18 |
| Flasks, pasteurized | ≈100 | 311 | 80.0 | 17.9 | 9.1 | 50.8 | 0.11 | 0.22 |
| B) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Culture mode | Glci (g/L) | Time (h) | Glccons (g/L) | X (g/L) | L (g/L) | YL/X (%,w/w) | YL/Glc (g/g) | YX/Glc (g/g) |
| Batch‐bioreactor | ≈50.0 | 72 | 44.5 | 12.7 | 8.1 | 63.8 | 0.18 | 0.29 |
| ≈100.0 | 160 | 90.9 | 25.2 | 14.2 | 56.3 | 0.16 | 0.28 | |
| ≈150.0 | 312 | 110.3 | 36.2 | 23.6 | 65.1 | 0.21 | 0.33 | |
Representation of initial glucose (Glci, g/L), consumed glucose (Glccons, g/L), produced biomass (X, g/L), produced lipid (L, g/L), lipid in dry weight (%, w/w), lipid yield per consumed substrate (YL/Glc, g/g), and biomass yield per consumed substrate (YX/Glc, g/g). Culture conditions for the shake flasks: growth in 250‐mL flasks at 185 rpm, initial pH = 6.0 ± 0.1, DO > 20% v/v, incubation temperature T = 26°C; for the bioreactor: agitation speed 200–500 rpm, pH = 6.0 ± 0.1, DO > 20% v/v, temperature T = 26°C. Two lots of independent cultures were conducted by using different inocula. In all of the determinations, standard error calculated was less than 10%.