Table 4.

Fatty acid composition of cellular lipids of Rhodosporidium toroloides DSM 4444 growing in shake flasks in previously pasteurized media presenting increasing initial glucose (Glc_i) concentration and constant NaCl concentration (A) and fatty composition of lipids of the same microorganism growing in batch‐bioreactor experiments in media presenting increasing initial glucose (Glc_i) concentration and constant NaCl concentration (B)

A)
Glci (g/L)	Growth phase	C14:0	C16:0	Δ9C16:1	C18:0	Δ9C18:1	Δ9,12C18:2	Δ9,12,15C18:3
≈50	VE	0.6	26.4	0.7	8.4	54.4	5.9	2.3
	L	1.1	23.0	0.5	7.9	57.4	6.9	2.6
≈100	VE	1.2	24.8	—	9.6	52.9	6.5	2.7
	L	1.6	22.0	1.5	8.2	55.0	6.8	2.2

B)
Glci (g/L)	Growth phase	C14:0	C16:0	Δ9C16:1	C18:0	Δ9C18:1	Δ9,12C18:2	Δ9,12,15C18:3
≈50	VE	1.5	25.2	—	12.0	50.2	8.4	2.0
	E	1.0	23.8	1.1	10.1	54.7	9.3	—
	L	1.2	22.1	1.5	10.8	55.7	6.5	1.4
≈100	VE	—	23.8	—	12.2	53.7	6.7	1.5
	E	—	22.1	2.5	11.9	55.4	5.8	2.0
	L	—	20.9	3.0	9.1	54.9	8.9	2.5

In the flask experiments, for Glc_i≈50 g/L, very early (VE) growth phase is that in which the incubation time is between 10 and 20 h and late growth phase is that in which incubation time is c. 150 h. For Glc_i≈100 g/L, VE growth phase is that in which the incubation time is between 20 and 30 h and late growth phase is that in which incubation time is c. 300 h. In the bioreactor experiments, for Glc_i≈50 g/L, VE growth phase is that in which the incubation time is between 10 and 20 h, early (E) growth phase is that in which the incubation time is between 30 and 40 h and late growth phase is that in which incubation time is c. 70 h. For Glc_i≈100 g/L, VE growth phase is that in which the incubation time is between 20 and 30 h, early (E) growth phase is that in which the incubation time is between 50 and 80 h and late growth phase is that in which incubation time is c. 150 h.