EGFP expression controlled by expression parts derived from two kinds of sequence sources. T, and HP sets were derived from C. glutamicum highly transcribed genes and B. subtilis highly expressed genes, respectively. Fluorescence intensity was normalized to OD600 of each construct. POS, positive control pXMJ19‐EGFP; NEG, negative control pE‐0; MEP and BEP, monocistronic and bicistronic expression parts, respectively. Numbers under horizontal axis indicate rank based on abundance of source genes.